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0.1 ug purified TKD was mixed with 27 uL of human sera and incubated at 37 C for different durations as indicated, the degradation of TKD was evaluated by western blotting assays, and the degradation curve was drawn using nonlinear regression based on the gray value. Input, purified TKD; F, sera derived from the female; M, sera derived from the male.
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