Temperature preference test of WT, Tmc6 –/– and Trpv1 –/– mice. n = 8 mice/group. Schematic diagram for the temperature preference test, the reference temperature (blue zone) was set at 35 °C, and the testing temperature (orange zone) was set at 50 °C ( h ) or 53 °C ( i ), the time mice spent in the testing zone within 5 min was counted.