Scatter Plot from Scientific Research

Mice were immunized in the footpad with OVA emulsified in IFA (IFA) or incorporated with CpG in nanoparticles (NP-CpG), as described in Fig. 1 , and the expression of selected genes by Tfh cells sorted 11 days after immunization was evaluated by RT-qPCR. The cells from 10 mice immunized with each adjuvant were pooled and tested in duplicate, both from C57BL/6 and Balb/c mouse strains. 2 − ΔCT values were determined in reference to the Actb housekeeping gene of the same sample and then normalized to the average 2 − ΔCT values obtained for mice immunized with the other adjuvant.

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