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Quantification of GC B cells (CD19 + CD95 + GL7 + ), Tfh cells (CD4 + Foxp3 - CD25 - CXCR5 + PD1 + ), and GC-Tfh cells (CD4 + Foxp3 - CD25 - CXCR5 hi PD1 hi ) in the draining LNs. Data from one experiment ( n = 4), each dot representing one sample and bars representing mean values, analyzed by one-way ANOVA and Tukeys multiple comparisons tests: ** P < 0.01, *** P < 0.001.
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