Normalized intracellular bacterial counts recovered from LPS-activated macrophages, with Ty2 WT in gray and Ty2 Δ mntR in blue. For complementation strains, YiiP ST4/74 is in orange, YiiP Ty2 is in green and YiiP(Δ2) ST4/74 is in red. Bars indicate the mean ± s.e.m. of the normalized bacterial count recovered from macrophages with individual values shown, derived from n = 5 biologically independent experiments. For all growth curves ( b – d , g ), each point and error bar indicates the mean ± s.e.m. of OD 600 , with reads taken at OD 600 once every 10 min. For f and h , significance was calculated using a one-way ANOVA; * P < 0.05, with multiple comparisons corrected by the Benjamini, Krieger and Yekutieli method (Supplementary Table 10 ).
