Top, correlation of gene fitness changes between (-) bile (LB only) and (+) bile (LB + 4% ox bile) in S . Typhi Ty2, with the acrAB/tol genes highlighted in red and all other genes shown in purple. Bottom, correlation of gene fitness changes between (-) polymyxin B and (+) polymyxin B in S . Typhimurium D23580, with LPS modification genes in red (including arn operon) and pmrABD genes highlighted in blue; all other genes shown in purple. For both panels in e , data are derived from n = 2 biologically independent Rb-Tn-seq replicates and shown as a density plot, where colors range from dark purple (low) to yellow (high), representing the kernel density estimation from low to high density.
