Colonic tissues from either untreated mice or mice administered 3% DSS for 7 days, as well as from mice fed AIN-76A or NWD1 synthetic diets for 3 months were analyzed for the presence of proliferative DCS cells. WGA was used to stain DCS cells 50 , and Ki67 to mark proliferative cells. Tissues were counterstained by DAPI (nucleus). Asterisks mark WGA/Ki67 double-positive cells.