Workflow from Scientific Research
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Cells are metabolically labeled in pulse chase experiments 9 followed by simultaneous sequencing. Pulse experiments involve incubation with nucleoside analogs for varying durations; in chase experiments, cellular mRNA fully incorporates nucleoside analogs during a long incubation, followed by washing out of these nucleosides for varying durations.
#Workflow#Illustration#Cells#Metabolic Labeling#Pulse-Chase Experiments#Sequencing#Nucleoside Analogs#mRNA
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