Microscopy from Scientific Research

Continuous illumination of a region of interest (5 x 5 m 2 ) of immobilized antibody-conjugated fluorophores in PBS using 640 nm excitation on a confocal microscope highlights photoblueing (left, fluorescence emission on 561 nm excitation) and photobleaching (middle, fluorescence emission on 640 nm excitation) for Abberior STAR RED, and changes in fluorescence lifetime for ATTO 647N (right, fluorescence lifetime on 640 nm excitation). Scale bars, 1 m.

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