Scatter Plot from Scientific Research

Simulations of smFRET efficiency for Cy3–Cy5 FRET pairs. Theoretical FRET efficiencies were calculated based on Forster theory without consideration of triplet states (Methods). Error bars in experimental FRET data represent s.d. in mean FRET efficiency values from five experimental repeats. The experimental data are crosstalk, direct excitation and gamma-corrected.

DOI