Scatter Plot from Scientific Research

Changes of γ -uncorrected ( e ) acceptor brightness (photon counts per 100 ms frames) for Cy3–Cy5 and LD555–LD655 FRET pairs attached to the DNA duplex (Fig. 2a ) with increasing illumination intensity. Data were collected in deoxygenated imaging buffers in the absence of any exogenous solution additives (photoprotective agents) at 100 ms time resolution using a custom-built TIRF imaging platform 51 . Error bars represent the s.d. of mean intensity values from five experimental repeats.

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