Heatmap from Scientific Research

Three-dimensional population FRET histograms (contour plots) of Cy3Cy5 FRET pairs attached to the DNA duplex at various illumination intensities, respectively. All the data were collected in deoxygenated imaging buffers in the absence of any exogenous solution additives (photoprotective agents) at 100 ms time resolution using a custom-built TIRF imaging platform 51. The data at 3.60 kW cm -2 were collected at 5 ms time resolution.

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