Line Plot from Scientific Research

smFRET measures distance within or between biomolecules that are site-specifically labeled with a donor and an acceptor fluorophore. When using Cy3 and Cy5 as donor and acceptor, FRET efficiency is most sensitive in the range ~30-70 A (left). An example of functionally important structural dynamics of an individual biomolecule that can be interrogated by smFRET imaging, illustrated by crystal structures of open and closed forms of the clam-shell-type amino acid sensor protein (LIV-BP) that interconvert upon leucine binding (middle) 10 . Green and red stars represent donor and acceptor fluorophores, respectively. Experimental smFRET trace of LIV-BP dynamics captured at high time resolution (1 ms) using wide-field TIRF imaging (right) 10 .

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