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Schematic overview of the experimental design. N represents the sample size. PE means the paired-end sequencing mode. SPF means specific pathogen-free. Fecal samples from the 1st–14th months post the cessation of antibiotic treatment were used for 16S rRNA gene sequencing. Fecal samples from the 12th month and 0.5–12th months were detected by metagenomics analysis and metabolomics analysis, respectively

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