Flow cytometry graphs, with the y-axis showing time in seconds and x-axis displaying fluorescence intensity (FI) units on a logarithmic scale. In the first column, events are plotted in 561–611/31 channel for detecting mCherry expression (above 550 Fi units), in the second column, events are shown in the 488–528/46 channel for detecting sYFP2 expression (above 500 Fi units), and in the third column, events are depicted in the 405–456/51 channel for detecting mTagBFP expression (above 450 FI units)