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Schematic of multiplexed assay of KCNE1 function. A comprehensively mutated barcoded plasmid pool was integrated into LP-KCNQ1-S140G cells. Cells were grown for 20 days and cell pools at 0 days, 8 days, and 20 days were deep sequenced. Different cell colors represent example KCNE1 variants (green: loss-of-function, blue: low function, gray: normal function, yellow: high function). Cells with functional KCNE1 variants were depleted from the cell pool over time.

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