Trafficking scores in the presence and absence of KCNQ1 were highly correlated with the exception of variants at residues 5, 7, 26, and 28 which disrupted glycosylation sites (highlight). Cysteine variants at these sites did not follow this trend (annotation). See Fig. S 5 for a full description of the KCNQ1 trafficking assay. Dotted lines: mean 1.96 SD for the synonymous distribution.