An HA tag was used to visualize KCNE1. Live HEK293T cells were stained with an Alexa Fluor 647-conjugated anti-HA antibody to detect surface KCNE1, then fixed, permeabilized, and stained with an Alexa Fluor 488-conjugated anti-HA antibody to detect total KCNE1. dsRed is the KCNQ1 and KCNE1 transfection marker. Cells expressing only KCNE1-HA showed minimal surface labeling in the absence of KCNQ1 (top). Cells expressing KCNE1-HA and KCNQ1 had normal cell surface expression (bottom).
