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Left: schematic of DNA attached at both ends with the approximate cut site location (AvrII, Eco53KI, and SacI). Right: fluorescence images showing PARP1-mEGFP at Eco53KI-induced blunt end DNA breaks and subsequent PARP1 removal by denaturing agents. DNA was visualized with Sytox Orange (10 nM) and GdnHCl (5 M) and urea (8 M) was used to denature the PARP1. Arrow indicates PARP1-DNA condensate.

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