Microscopy from Scientific Research

Fluorescence images showing that Alexa 647N-PARP1 (25 nM) rescues an Eco53KI-induced double-strand break and recruits FUS-mEGFP (100 nM) in an NAD+(0.15 mM)-dependent manner. Arrows indicate PARP1-DNA condensates on the DNA. DNA visualized with Sytox Orange (10 nM). Scale bars: 2 m. See alsoFigure S7.

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