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Schematic representation of the selection of timepoints for the ribo-seq experiment. Thus, high Ftl1 , Fth1 and Alas2 translation efficiency and low Tfrc abundance, conceivably as a result of mRNA instability, occur in the light phase of the day, leading us to predict low IRP activity around ZT5. By contrast, low Ftl1 , Fth1 and Alas2 translation efficiency and the rise in Tfrc abundance at the lightdark transition suggest high IRP activity at ZT12.
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