Left: workflow. Middle: fluorescence images of PARP1-mEGFP (1 M) with Cy3-labeled 45 bp dsDNA (2 M), NAD+(0.2 mM). Incubation for 30 min at 25°C. Right: the fraction of condensed PARP1 as a function of NAD+concentration. Repeated three times with similar results. Circle size proportional to the fraction condensed, x denotes no condensates.
