Schematic representation of Nexilin expression constructs. (Upper) Full length of Nexilin with 671 amino acids (aa) consists of a central coiled-coil (CC) domain flanked by two actin-binding domains (ABD) and a highly basic C-terminal IGcam domain. S1 (1-465aa) is a shortened version that removes C-terminal Igcam-containing region. S2 (168-671aa) deletes the first ABD domain. S3 (299-671aa) consists of the second ABD domain and IGcam domain. S4 (466-671aa) only contains C-terminal domain with IGcam. Key mutations within the coding region of NEXN gene identified from DCM patients are marked. (Lower) Schematic diagram of AAV construct containing cTnt promoter-driven different segments of mNexn variants.
