An upset plot for autosomal genes that were found significantly differentially expressed between males and the three groups of female lines. The upset plot is cropped to display the 10 largest overlapping gene groups (excluding 549 genes). The full plot is displayed in Additional file 1 : Fig. S7A. The horizontal bar graph displays the total number of female- and male-biased genes for each group. The horizontal bars are colored by the female group: pink for group 3, violet for group 2, and red for group 1. The boxplots below the upset plot present the mean standardized gene expression for male lines (M) and the group 1 (G1), group 2 (G2), and group 3 (G3) female lines for subsets of overlapping genes. The vertical bars indicate the sharing of significant male–female differences between the female groups. The unique effects in group 3 are colored in pink, the effects shared by group 2 and group 3 are colored in violet and outlined with pink, the unique effects in group 2 are colored in violet without outline, and the effects that are unique for group 1 are colored in red. The non-concordant effects are cropped out (see Additional file 1 : Fig. A).
