The relationship of XIST expression and fraction of X chromosome genes with bi-allelic expression (ASE > 0.1, one-sided binomial test, FDR q -value < 0.01). K-means clustering of XIST level and fraction of genes with ASE > 0.1 identifies three groups of female lines. group 1: high- XIST female lines with low level of bi-allelic expression (red, n = 74), group 2: intermediate female lines that express XIST but have high fraction of de-repressed genes (violet, n = 45). Group 3: low- XIST females with high fraction of de-repressed genes (pink, n = 46). The vertical dashed line indicates the threshold for XIST expression level which was used to define low- and high-XIST female groups earlier.
