A plot of pratio (defined as the ratio of the exact Wilcoxon p -value, pexact , to the asymptotic Wilcoxon p -value, pasymptotic ) vs. goodness of fit of the Gaussian distribution to the exact null distribution (as quantified by the KS statistic). Each point represents a gene-gRNA pair; pairs 1 and 2 (from panel a ) are annotated. As the KS statistic increases (indicating worse fit of the Gaussian distribution to the exact Wilcoxon null distribution), pratio deviates more from one, indicating miscalibration. Points are colored according to the effective sample size of the corresponding pair.
