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The standard paradigm for association testing on low-MOI single-cell CRISPR screen data. To test for association between a given targeting perturbation and gene, one tests for differential expression of the gene across two groups of cells: those containing the given targeting perturbation and those containing a non-targeting (NT) perturbation. One typically repeats this procedure for a large, preselected set of targeting-perturbation gene pairs, obtaining a discovery set by subjecting the resulting p -values to a multiple comparison correction procedure (e.g., Benjamini-Hochberg).

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