Schematic overview depicting the bead groups (KT: negative control with capped groups and no capturing mAb, Cy3: capturing anti-EphA2 mAb, Cy5: anti-EpCAM mAb), protein groups (1: no protein added to the beads, 2: 1 ng of EphA2 protein, 3: 1 ng of EpCAM protein and 4: 1 ng of EphA2 and EpCAM protein each), and expected results (positive binding beads result in a white color on merged composite images).
