Workflow from Scientific Research

Open access visualization of Workflow, Mutagenesis, C. elegans, Oxygen Levels, Genetic Screen
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DOI: 10.1016/j.cell.2023.12.010

DOI

gas-1(fc21) or nduf-7(et19) P0 animals were randomly mutagenized with ethyl methanesulfonate (EMS) at 21% oxygen. F2 progeny were transferred to the non-permissive 50% oxygen tension and selected for growth. NDUFS4/lpd-5 null C. elegans are not fertile at any oxygen tension, making a forward genetic screen challenging.

#Workflow#Mutagenesis#C. elegans#Oxygen Levels#Genetic Screen

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