Extent of Coordinator-motif-binding preference of V5-tagged TWIST1 and various loop mutants expressed in HEK293 cells (seeFigure S6A for protein levels) with or without ALX4. Inset: structural alignment of TWIST1 in our structure with the AlphaFold-predicted (AF) or experimentally solved (PDB:2QL2and2YPA) structures of tested bHLH loops.
