Flow cytometry analysis of tumor-infiltrating CD4 + and CD8 + T cell percentages within the CD45 + pool of MC38 tumors from Depdc5 ncl and Depdc5 tko mice treated with vehicle or DFO. At day 23 after tumor inoculation, single-cell suspensions of each tumor were isolated from individual mice, then stained with a fixable viability dye and antibodies against CD45 and CD8 prior to analysis by flow cytometry.
