Live imaging of EB3 comet in HeLa cells expressing TubAR (e), siTUBA1A (g), and siTUBB4A (i) visualized by viewing successive frames as different channels, red, green, and blue, creating a rainbow effect. More rapid polymerizations result in a longer rainbow with less overlap between the colors and slower polymerizations result in a shorter rainbow with greater overlap in the colors manifested as white. The images were pooled from plus-end microtubule binding assay. Representative videos of EB3 tracking behavior can be seen in Supplementary movies.
