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Quantification of EB3 comet velocity of TubAR ( f ), TUBA1A ( h , knockdown), and TUBB4A ( j , knockdown) on the microtubule assembly dynamics in HeLa cells. TubAR antisense ( TubAR AS ), serving as a negative control to TubAR ; n number of cells pooled from three independent experiments. Data are represented as means ± SEM. Statistical significance was determined using unpaired t -test with Welch’s correction ( f , h , j ); *** P < 0.001.

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