Line Plot from Scientific Research

Binding affinity for the wild type (WT) and selected mutants with BRV measured using microscale thermophoresis assay ( K d (μM): WT, 0.809 ± 0.194; W300A, 26.03 ± 7.3; Y461A, 3.78 ± 1.4; W666A, 41.2 ± 1.3; D670A, 1.23 ± 0.38; mean ± SEM, n = 3–4 independent experiments).

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