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Representative confocal images of Ptdlns3P and ZFYVE1-GFP puncta in WT and KO-IFRD1 PLC/PRF/5 cells cultured with normal or glutamine-free medium for 48 h (left). The numbers of Ptdlns3P and ZFYVE1-GFP puncta were quantified per cell ( n = 5) (right). Scale bars, 20 m. Data shown are representative of three independent experiments. Data are mean SD; * P < 0.05; ** P < 0.01 by two-way ANOVA ( g , h ).
#Bar Plot#Error Bars#Microscopy#Confocal Images#Ptdlns3P#ZFYVE1-GFP#Wild-Type#KO-IFRD1#PLC/PRF/5 Cells#Glutamine#Puncta Quantification
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