Microscopy from Scientific Research

Representative electronic micrographs of autophagosomes or autolysosomes of WT and KO-IFRD1 PLC/PRF/5 cells under glutamine starvation conditions for 36 h. Red arrows indicate autophagic structures. Insets: magnified views of the regions in the white boxes (left). The number of autophagic structures per area was quantified ( n = 6) (right). Scale bars, 1 μm. Data shown are representative of three independent experiments. Data are mean ± SD. ** P < 0.01, *** P < 0.001; ns, not significant by two-tailed unpaired Student’s t test or two-way ANOVA.

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