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Representative images of LC3B immunostaining in WT and KO-IFRD1 PLC/PRF/5 cells under glutamine starvation conditions for 36 h with the addition of DMSO or Baf-A1 (400 nM) for the last 2 h (left). DAPI staining indicates nucleus. Percentage of LC3B puncta per cell in WT and KO-IFRD1 PLC/PRF/5 cells (glutamine-starved for 36 h) with Baf-A1 was quantified ( n = 5) (right). Scale bars, 20 μm.
#Bar Plot#Microscopy#Photo#Error Bars#LC3B Immunostaining#WT Cells#KO-IFRD1 Cells#PLC/PRF/5 Cells#Glutamine Starvation#DMSO#Baf-A1#DAPI#LC3B Puncta
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