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Spectra of the mixture of AIF, N-pep, and NAD+(12.1 μM, 139 μM, and 6.24 mM, respectively, in 50 mM Tris-HCl, pH 8.0) at 30°C (black), 25.5°C (orange), 23.5°C (dark blue), 21.5°C (brown), 20°C (red), 18°C (purple), 16°C (light green), 14°C (green) 10°C (light blue), and 5°C (blue).
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