Representative images of dopaminergic neurons (DAn) from control-hiPSC and LRRK2-PD hiPSC co-stained for tyrosine hydroxylase (TH, red marker of dopaminergic neurons), DAPI (blue marker of cell nucleus) and -synuclein (green), treated with M324 (20 M). In the upper panel, all the three markers are displayed, while in the bottom panel only -synuclein and DAPI are displayed to highlight the accumulation of -synuclein in LRRK2-PD DAns treated with vehicle, and the absence of accumulation in DAns treated with M324 (20 M).
