Kinome trees showing the experimentally confirmed kinase hits at 10 M concentration (red and green dots) for rucaparib (data derived from ref.15) and M324 (data from this work). A threshold of reducing kinase activity below 50% was applied to define a kinase hit. Kinases inhibited by more than 90% by M324 are shown in green. Circle size is proportional to the percentage of inhibition, 100% inhibition being the largest dots. White circles represent kinase hits of M324 that were not tested in rucaparib, or vice versa. Staurosporine was used as a control in most of the assays.
