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PBMCs were transfected with non-targeting control or UCH-L1-specific siRNA and stimulated with LPS for 4 h and nigericin for different times as indicated. Production of IL-1beta in the supernatants from PBMCs as measured by ELISA. Error bars show the standard deviation of the mean.*p< 0.05,**p< 0.01 (unpaired two-sided t test). The results are representative of 3 independent experiments.
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