Immunoblot and RT-qPCR analysis of whole-cell lysates and supernatants from THP-1 cells transfected with non-targeting control siRNA or UCH-L1-specific siRNA. Cells were differentiated with PMA and primed with LPS (1 ug/mL) for 4 h, followed by treatment with nigericin (10 uM) for different times as indicated. Error bars show the standard deviation of the mean.
