Immunoblot of cell lysates from HMC3 cells transfected with non-targeting control siRNA or UCH-L1-specific siRNA. Cells were primed with LPS (1 ug/mL) for 4 h, followed by treatment with nigericin (10 uM) for 45 min. IL-1beta bands were quantified using GelAnalyzer and normalized using beta-actin bands. Error bars show the standard deviation of the mean.**p< 0.01 (unpaired two-sided t test).
