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Image processing pipeline of NLRP3 puncta detection and quantitative analysis. Confocal images were first flat field corrected, followed by the segmentation of nuclei and cytoplasm. NLRP3 puncta were identified, counted, and analyzed using the Columbus Image Data Analysis System. Scale bars, 10 μm.
#Workflow#Microscopy#Image Processing#NLRP3 Puncta#Quantitative Analysis#Confocal Images#Nuclei Segmentation#Cytoplasm Segmentation#Columbus Image Data Analysis System
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