In vitro validation: CRISPR-Cas9 depletion time course showing relative growth defects introduced by gRNAs against PTK2 (purple), ITGAV (blue), the negative control AAVSI (red), or the positive control gRNA POLRA (green). gRNA-containing GFP+ cell numbers of weekly measurements over 24 days were normalized to the GFP+ cell numbers of day 3 upon transduction with the gRNAs. Mean and standard deviation of three biological replicates are shown. T-47D-resistant cell line data are from two biological replicates and with only one biological replicate for the day 10 measurement.
