The maps of the group IIB RNP were generated using a tilted data collection strategy with the stage set to 30. The map on the left was globally refined and shown at both a high (teal) and low threshold (white). The group II intron RNP displays clear RNA features at high threshold and density for the embedded RNA is observed at low threshold. Performing local refinement on the density corresponding to the embedded RNA yielded the map on the left (gold) which has been overlaid on the low threshold globally refined map (white) for clarity.
