Kymographs of TIRF time-lapse colocalization between CLC2-mRuby3 and SH3P2-GFP in the epidermis of the root early elongation zone (C) and of etiolated hypocotyls (D). SH3P2-GFP is recruited at the end of some instances of CCV formation (yellow arrowheads). In (C), the completed CCV with SH3P2-GFP can be seen departing from the PM to the left in the plane of section before the signal disappears, presumably due to vesicle uncoating. Many captured CCPs do not recruit SH3P2-GFP (white arrowheads). SH3P2-GFP is also recruited at sites not containing clathrin (blue arrowheads). See also Figure S6B.
