TIRF images of CLC2-GFP in the hypocotyl epidermis of the wild type and sh3p12310G. Scale bar, 2 m. The graph shows quantifications of CLC2-GFP-positive focus density, with each data point representing one cell. Wild type: 74.2 14.2 foci per 100 m2 (mean SD), n= 18; sh3p12310G: 77.9 11.5 foci per 100 m2, n= 18. Values were compared using a t test.