TernarycisSNARE species are converted to syntaxin-Munc18 complexes via NSF-α-SNAP-mediated disassembly driven by ATP. Synaptic vesicles may then associate and move to a fusion-ready state, which may finally undergo Ca2+-dependent fusion. α-SNAP antagonizes the generation of fusion-ready synaptic vesicles. Munc13, and not just the MUN domain, in turn inhibits the actions of α-SNAP/NSF to promote fusion.
