Quantification of BrdU incorporation in neonatal (postnatal day 1, P1) cardiomyocytes upon treatment with inhibitors of AKT (Akt inhibitor VIII 0.5 μM) or ERK (PD0325901 0.2 μM), with or without administration of BMP7 at 10 ng/mL for 48 h (n= 8204 cardiomyocytes pooled from the analysis of 31 samples); cardiomyocytes were identified by cardiac Troponin I (cTnI) immunostaining. Representative pictures are provided; arrows point at proliferating cardiomyocytes; scale bars, 20 μm.
